Insulin Binding to Liver Cells: A Simple and Useful In Vivo Model

J. J. Gagliardino; M. T. Pessacq; D. Turyn; J. M. Dellacha

doi:10.1055/s-2007-996275

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 1980; 12(7): 300-303
DOI: 10.1055/s-2007-996275

ORIGINALS

Insulin Binding to Liver Cells: A Simple and Useful In Vivo Model

J. J. Gagliardino¹ , M. T. Pessacq¹ , D. Turyn² , J. M. Dellacha²

¹Centro de Endocrinología Experimental y Aplicada (CENEXA), UNLP-CONICET: Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina
²Departamento de Química Biológica. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires and Centro para el Estudio de las Hormonas Hipofisarias, Buenos Aires, Argentina

Abstract

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Summary

Insulin binding to rat liver cells was studied in vivo, by I.V. injection of (¹²⁵I)-labelled hormone either alone or together with different amounts of unlabelled insulin.

Native insulin competes with the tracer in a manner similar to the one observed in the in vitro preparation. Prolactin, bovine growth hormone or glucagon were unable to displace labelled insulin from its binding sites.

The hypoglycemic effect of various amounts of native insulin was measured and correlated to the binding studies.

It was found that maximal insulin-induced hypoglycemia occurs at an insulin concentration at which only about 12 % of the measurable receptors are occupied.

Key-Words

Receptors - Insulin - Hypoglycemia - Liver Receptors

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